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human wild type retinal pigment epithelial cells  (ATCC)


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    ATCC human wild type retinal pigment epithelial cells
    Human Wild Type Retinal Pigment Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human wild type retinal pigment epithelial cells/product/ATCC
    Average 99 stars, based on 4645 article reviews
    human wild type retinal pigment epithelial cells - by Bioz Stars, 2026-03
    99/100 stars

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    human wild type retinal pigment epithelial cells  (ATCC)
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    A. <t>Wild</t> <t>type</t> RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.
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    A. <t>Wild</t> <t>type</t> RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.
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    wild type human retinal pigment epithelial cells  (ATCC)
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    ATCC wild type human retinal pigment epithelial cells
    A. <t>Wild</t> <t>type</t> RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.
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    https://www.bioz.com/result/wild type human retinal pigment epithelial cells/product/ATCC
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    A. Wild type RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.

    Journal: bioRxiv

    Article Title: Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites

    doi: 10.1101/2025.07.29.667472

    Figure Lengend Snippet: A. Wild type RPE1 cells immunostained against Sec31A. B. Scatter plot of number of Sec31A positive structures in wild type RPE1 cells, colour coded by experimental repeat, n = 32 cells. C. HaloTag-Sec23A-RPE1 cells dyed with JFX650-HaloTag ligand (green) and co-stained using an antibody directed against Sec31A (purple). Inset highlights tight colocalization of HaloTag-Sec23A fusion protein and endogenous Sec31A. D. Example cryo-fluorescence wide field image of HaloTag-Sec23A-RPE1 cells grown on grids stained with Oregon Green HaloTag ligand before and after milling. HaloTag-Sec23A displays a punctate staining pattern throughout the cytoplasm and is concentrated at the perinuclear region. The red box in the left panel indicates the digitally magnified region in the other panels which show cryo-fluorescence before (middle panel) and after (right panel) milling. Four cells are indicated by green dotted outlines. Blue arrow heads indicate clusters of fluorescent fiducials. Green arrows indicate fluorescent puncta visible both before and after milling. Scale bars: 10um, inset in panel C: 1um.

    Article Snippet: Wild type human retinal pigmented epithelial cells (RPE-1) were obtained from ATCC and were cultured in DMEM/F12 (ThermoFisher Scientific) plus 10% FBS, L-glutamine and 1% Pen/Strep (Bio Basic).

    Techniques: Staining, Fluorescence